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The aim of this study is to conduct a knowledge acquisition through the analysis of backscattering and reflecting properties of different urban land cover types using TerraSAR X-band data and a multispectral Quickbird image, and propose an appropriate technique for the acquired knowledge representation. The backscatter and reflectance values of different land features are compared on the basis of statistics of signatures of the selected classes. Overall, the study demonstrated that the TerraSAR and Quickbird images can be successfully used for the knowledge acquisition as well as for the investigation of different land surface features based on spectral analysis.